diff --git a/src/wp-admin/includes/upgrade.php b/src/wp-admin/includes/upgrade.php
index 705539571e..cb3939108b 100644
--- a/src/wp-admin/includes/upgrade.php
+++ b/src/wp-admin/includes/upgrade.php
@@ -843,6 +843,10 @@ function upgrade_all() {
 		upgrade_640();
 	}
 
+	if ( $wp_current_db_version < 57155 ) {
+		upgrade_650();
+	}
+
 	maybe_disable_link_manager();
 
 	maybe_disable_automattic_widgets();
@@ -2349,6 +2353,35 @@ function upgrade_640() {
 	}
 }
 
+/**
+ * Executes changes made in WordPress 6.5.0.
+ *
+ * @ignore
+ * @since 6.5.0
+ *
+ * @global int  $wp_current_db_version The old (current) database version.
+ * @global wpdb $wpdb                  WordPress database abstraction object.
+ */
+function upgrade_650() {
+	global $wp_current_db_version, $wpdb;
+
+	if ( $wp_current_db_version < 57155 ) {
+		$stylesheet = get_stylesheet();
+
+		// Set autoload=no for all themes except the current one.
+		$theme_mods_options = $wpdb->get_col(
+			$wpdb->prepare(
+				"SELECT option_name FROM $wpdb->options WHERE autoload = 'yes' AND option_name != %s AND option_name LIKE %s",
+				"theme_mods_$stylesheet",
+				$wpdb->esc_like( 'theme_mods_' ) . '%'
+			)
+		);
+
+		$autoload = array_fill_keys( $theme_mods_options, 'no' );
+		wp_set_option_autoload_values( $autoload );
+	}
+}
+
 /**
  * Executes network-level upgrade routines.
  *
diff --git a/src/wp-includes/version.php b/src/wp-includes/version.php
index 741a527c5e..ecd665df63 100644
--- a/src/wp-includes/version.php
+++ b/src/wp-includes/version.php
@@ -23,7 +23,7 @@ $wp_version = '6.5-alpha-56966-src';
  *
  * @global int $wp_db_version
  */
-$wp_db_version = 56657;
+$wp_db_version = 57155;
 
 /**
  * Holds the TinyMCE version.